©2013 Elchrom Scientific AG | all rights reserved | Gewerbestrasse 8 | CH-6330 Cham | Switzerland | +41 41 747 25 50

EnhanceIT Polymer Solution

"-" : without EnhanceIT Polymer Solution, "+" : with EnhanceIT Polymer Solution

Product Flyer

EnhanceIT Polymer Solution is a supplement for regular polyacrylamide (paa) gels for DNA separation. The chemical properties of EnhanceIT alter the characteristics of paa gels such that their resolving power is substantially increased, resulting in much shorter gels and running times. A 1 bp resolution can be achieved on 8 cm running distance; 4 bp on 4 cm!

Using shorter paa gels has several advantages: the gels are easier to prepare and handle, they allow faster runs, and shorter gels will consume less staining reagents. In addition, due to the higher resolving power of paa gels including EnhanceIT, hence the shortened running distance, the bands appear sharper and less DNA is required. Consequently, EnhanceIT gels have a higher sensitivity.

Ordering Information

P/NProductQuantity
3551EnhanceIT Polymer Solution 50 ml50 ml
3552EnhanceIT Polymer Solution 250 ml250 ml
3553EnhanceIT Polymer Solution 500 ml500 ml
3554EnhanceIT Polymer Solution 1000 ml1000 ml

Markers

M3 Marker for Spreadex® Gels

The M3 Marker was specially designed for precise size determination of unknown DNA fragments on Spreadex® gels. The complexity of the marker provides both, a suitable number of reference fragments for each Exclusion Limit (EL 300 to 1200) and its separation pattern matches the resolving power of Spreadex® gels. The marker contains over 50 DNA fragments in the size range from 50 to 622 bp. 

The fragments were produced by HaeIII digestion of pBR322 (blunt end), and Mspl and Hhal cleavage (overhangs). On Spreadex® gels, all the fragments migrate in the expected order according to their length.

M1 Marker

The M1 Marker was optimised for precise size determination of unknown DNA fragments on GMA gels, 12% and 9% Poly(NAT)® gels, as well as PCR CheckIT™ gels.

DNA fragments were obtained by digestion of pBR322 with MspI restriction enzyme. 

Technical specifications for M1 and M3:

Volume 50 µl
Loading 1 µl for EtBr staining
0.2 µl for SYBR stained gels
Storage -20° C


Ready-To-Use Markers

Pre-diluted M1 and M3 Marker for immediate use: no dilution, no dyes, no loading buffer required, simply load on a gel.

Technical specifications for Ready-To-Use Markers:

Volume 50 µl
Loading 4 to 5 µl for both, EtBr and SYBR staining.
Storage -20° C

Ordering Information

P/NProductQuantity
3203M3 Marker 50 loadings
3204M1 Marker 50 loadings
3203EBReady-to-use M3 Marker for EtBr staining50 µl
3203SYReady-to-use M3 Marker for SYBR staining50 µl
3204EBReady-to-use M1 Marker for EtBr staining50 µl
3204SYReady-to-use M1 Marker for SYBR staining50 µl

Buffers

  • Running Buffer
  • Sample Loading Buffer
  • PCR Loading Buffer
  • 0.2M TMAP (tetramethylammonium phosphate)
  • Solid Phase Extraction Buffer
  • Oligo Electroelution Buffer

 

Ordering Information

P/N  ProductQuantity
303140 x Running Buffer Stock Solution, 1.2 M TAE buffer 1 l (20x50ml)
3033    Sample Loading Buffer 10 ml  10 x 1 ml
3034Sample Loading Buffer 50 ml  50 x 1 ml
3035Sample Loading Buffer (2 dyes, BPB and XC)10 x 1 ml
3038TMAP, 0.2 M1 ml
9008SPE, Solid Phase Extraction Washing Buffer

1 L

9009Oligo Electroelution Buffer 1 L


Destaining Solution

After incubation in a solution containing an intercalating dye, DNA gels are usually destained in water. The Elchrom Scientific destaining solution speeds up the destaining process and reduces gel background. This is particularly eminent in the case of Spreadex®  EL 300-500 gels and 12% Poly(NAT)® gels if stained with SYBR Green or SYBR Gold.

Typically, gels are destained for 20 - 30 min.

The destaining solution is available as a 100 x concentrate.

Ordering Information

P/NProduct Quantity
3037DST, Destaining Solution 100x 50 ml


websign by multipol