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EnhanceIT Polymer Solution is a supplement for regular polyacrylamide (paa) gels for DNA separation. The chemical properties of EnhanceIT alter the characteristics of paa gels such that their resolving power is substantially increased, resulting in much shorter gels and running times. A 1 bp resolution can be achieved on 8 cm running distance; 4 bp on 4 cm!
Using shorter paa gels has several advantages: the gels are easier to prepare and handle, they allow faster runs, and shorter gels will consume less staining reagents. In addition, due to the higher resolving power of paa gels including EnhanceIT, hence the shortened running distance, the bands appear sharper and less DNA is required. Consequently, EnhanceIT gels have a higher sensitivity.
|3551||EnhanceIT Polymer Solution 50 ml||50 ml|
|3552||EnhanceIT Polymer Solution 250 ml||250 ml|
|3553||EnhanceIT Polymer Solution 500 ml||500 ml|
|3554||EnhanceIT Polymer Solution 1000 ml||1000 ml|
M3 Marker for Spreadex® Gels
The M3 Marker was specially designed for precise size determination of unknown DNA fragments on Spreadex® gels. The complexity of the marker provides both, a suitable number of reference fragments for each Exclusion Limit (EL 300 to 1200) and its separation pattern matches the resolving power of Spreadex® gels. The marker contains over 50 DNA fragments in the size range from 50 to 622 bp.
The fragments were produced by HaeIII digestion of pBR322 (blunt end), and Mspl and Hhal cleavage (overhangs). On Spreadex® gels, all the fragments migrate in the expected order according to their length.
The M1 Marker was optimised for precise size determination of unknown DNA fragments on GMA gels, 12% and 9% Poly(NAT)® gels, as well as PCR CheckIT™ gels.
DNA fragments were obtained by digestion of pBR322 with MspI restriction enzyme.
Technical specifications for M1 and M3:
Volume 50 µl
Loading 1 µl for EtBr staining
0.2 µl for SYBR stained gels
Storage -20° C
Pre-diluted M1 and M3 Marker for immediate use: no dilution, no dyes, no loading buffer required, simply load on a gel.
Technical specifications for Ready-To-Use Markers:
Volume 50 µl
Loading 4 to 5 µl for both, EtBr and SYBR staining.
Storage -20° C
|3203||M3 Marker||50 loadings|
|3204||M1 Marker||50 loadings|
|3203EB||Ready-to-use M3 Marker for EtBr staining||50 µl|
|3203SY||Ready-to-use M3 Marker for SYBR staining||50 µl|
|3204EB||Ready-to-use M1 Marker for EtBr staining||50 µl|
|3204SY||Ready-to-use M1 Marker for SYBR staining||50 µl|
|3031||40 x Running Buffer Stock Solution, 1.2 M TAE buffer||1 l (20x50ml)|
|3033||Sample Loading Buffer 10 ml||10 x 1 ml|
|3034||Sample Loading Buffer 50 ml||50 x 1 ml|
|3035||Sample Loading Buffer (2 dyes, BPB and XC)||10 x 1 ml|
|3038||TMAP, 0.2 M||1 ml|
|9008||SPE, Solid Phase Extraction Washing Buffer|
|9009||Oligo Electroelution Buffer||1 L|
After incubation in a solution containing an intercalating dye, DNA gels are usually destained in water. The Elchrom Scientific destaining solution speeds up the destaining process and reduces gel background. This is particularly eminent in the case of Spreadex® EL 300-500 gels and 12% Poly(NAT)® gels if stained with SYBR Green or SYBR Gold.
Typically, gels are destained for 20 - 30 min.
The destaining solution is available as a 100 x concentrate.
|3037||DST, Destaining Solution 100x||50 ml|
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