Overview Of Different Gels: Size Range & Resolution

Type of Gel	Optimal Size Range bp	Resolution	Special Feature
Spreadex^®	50-1000	1 - 4 bp	highest resolving power
EL 1200	250-800	4 bp
EL 800	200-500	4 bp
EL 600	150-350	4 bp
EL 500	100-300	4 bp
EL 400	65-250	1 bp*
EL 300	50-200	1 bp*

PCR ChekIT^TM	100-4000	10 bp	fast runs and easy DNA recovery

GMA	100-450	1 bp	SSCP and heteroduplex analysis

Clearose^® RNA	500-10000 nt		RNA analysis

Poly(NAT)^®	50-20000	2 -3%**	fine separations in broad size ranges
6%	400-20000
9%	180-800
12%	60-300

Short Fragment^TM	<50	1 bp/nt	For high resolution analysis of short nucleic acids

* on 8 cm running distance

** for fragments up to 2 kb

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Type of Gels	Application
Spreadex^®	Microsatellite Analysis RFLP (Restriction Fragment Length Polymorphism) >Application Note: High throughput ApoE Multiplex PCR Competitive PCR Allele specific PCR RNA Analysis GMO Analysis

PCR CheckIT^TM	PCR Checking High Throughput PCR Analysis DNA recovery RFLP (Restriction Fragment Length Polymorphism) HLA Typing (Human Lymphocyte Antigen Typing) >Application Note: HLA Typing

GMA	SSCP (Single Strand Conformational Polymorphism) >Application Note: Cold SSCP _Cryptosporidium >Application Note: SSCP _Cytochrome Heteroduplex Analysis

Clearose^® RNA	Easy for Northern Blotting Qualitative RNA analysis Analysis of RNA transcripts mRNA and tRNA 500 nt - 10000 nt

Poly(NAT)^®	RFLP (Restriction Fragment Length Polymorphism) RAPD (Randomly Amplified Polymorphic DNA) >Application Note: RAPD fingerprinting of B. japonicum

Short Fragment^TM	Oligonucleotide analysis siRNA analysis

Elchrom's ready-to-use gels are available in 8 different formats. They allow the analysis of up to 400 samples per gel.

Elchrom provides two main formats of gels:

Mini Gels: 8.7 cm running distance for 8 samples or 5.7 cm for 12 samples.
Wide Mini Gels:
8.7 cm running distance for 2 x 13 and 2 x 25 samples
4 cm funning distance for 4 x 13 and 4 x 25 samples
17 mm running distance for 2 x 104
8 mm running distance for 2 x 104L and 2 x 200

Wide Mini Gels have either 2 or 4 gels per presentation.

General Features:

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